immunology & Biochemistry
Soheyla Yadamani; Ali Neamati; Masoud Homayouni Tabrizi; Samira Yadamani
Volume 24, Issue 5 , September and October 2018, , Pages 375-380
Abstract
Background: Schiff bases have attracted great attention in recent years due to their antioxidant, antiviral and antibacterial properties. Antioxidants prevent free radical reactions and leading to decrease in cardiovascular disease, cancers, etc. Bacteria exploited variety of defense systems so they ...
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Background: Schiff bases have attracted great attention in recent years due to their antioxidant, antiviral and antibacterial properties. Antioxidants prevent free radical reactions and leading to decrease in cardiovascular disease, cancers, etc. Bacteria exploited variety of defense systems so they can resist against antibiotics. Therefore, it is necessary to develop antimicrobial factors with more effective mechanisms. In present study, antioxidant and antimicrobial properties of copper Schiff base complex N’-N1 dipirodoxil-1-2 diaminobenzene has been studied. Materials and methods: After Synthesis of Schiff base complex, evaluation of antiradical activity of this complex was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH),2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and hydroxyl (OH ̊) tests. In order to study antimicrobial properties, disk diffusion test was done using bacteria.Results: Schiff base complex acted as scavengerof 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) (IC50 = 0.1 mg/ml), 2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) (IC50 = 0.11 mg/ml) and hydroxyl radicals (OH ̊) (IC50 = 0.14 mg/ml). Antimicrobial effects of this complex against two bacteria were seen at the concentration of 250 µg/ml.Conclusion: Findings of this study indicate that copper Schiff base complex has antioxidant activity and in antimicrobial test Staphylococcus aureus is more sensitive than Escherichia coli. Keywords: Copper Schiff base; Antiradical Activity; DPPH; Antimicrobial